Several Affinity Tags Commonly Used in Chromatographic Purification

Several Affinity Tags Commonly Used in Chromatographic Purification

Affinity tags have become powerful tools from basic biological research to structural and functional proteomics. They were widely used to facilitate the purification and detection of proteins of interest, as well as the separation of protein complexes. Here, we mainly discuss the benefits and drawbacks of several affinity or epitope tags frequently used, including hexahistidine tag, FLAG tag, S...

Commonly used tag combinations for tandem affinity purification.

TAP (tandem affinity purification) allows rapid and clean isolation of a tagged protein along with its interacting partners from cell lysates. Initially developed in yeast, the TAP method has subsequently been adapted to other cells and organisms. In combination with MS analysis, this method has become an indispensable tool for systematic identification of target-associated protein complexes. T...

RNA affinity tags for purification of RNAs and ribonucleoprotein complexes.

Intrinsic affinity tags are useful tools for the study of macromolecular targets. Although polypeptide affinity tags are routinely used in purification and detection of protein complexes, there has been a relative lack of powerful RNA affinity tags that can be embedded within RNA sequences. Here, the preparation and use of two RNA affinity tags against Sephadex or streptavidin are described. Th...

Chromatographic Purification of MAbs with Non-Affinity Supports

Deposition of carbon nanotubes in commonly used sample filter media

There is no single standard technique or methodology to characterize the size, structure, number, and chemical composition of airborne carbon nanotubes.  Existing analytical instruments and analytical techniques for evaluating nanoparticle concentrations cannot simultaneously provide morphology, state of agglomeration, surface area, mass, size distribution and chemical composition data critical...